TITLE:
Analytical Optimization of GeneXpert Ultra for Detection of Tuberculosis in CSF Samples
AUTHORS:
Victoria L. Campodónico, Derek T. Armstrong, Gene Olinger, Erin A. Merritt, Ryan Howard, Margaret Megan Lemmon, Nikki Parrish
KEYWORDS:
GeneXpert Ultra, Tuberculous Meningitis, Mycobacterium tuberculosis, CSF
JOURNAL NAME:
Journal of Tuberculosis Research,
Vol.11 No.3,
September
26,
2023
ABSTRACT: Detection
of Mycobacterium tuberculosis complex (MTBC) in extrapulmonary specimens can be challenging
due to their paucibacillary nature. This difficulty is especially true for
cerebrospinal fluid (CSF), and the low sensitivity and specificity for
diagnosis hampers rapid detection and treatment in vulnerable populations.
GeneXpert MTB/RIF Ultra has been shown to provide rapid results for detection
of MTBC and associated Rifampin resistance, but there is limited data regarding
standardized methods for CSF processing on this assay. In this study, we sought
to provide guidance regarding the best methods for CSF processing, including
optimal volumes to test, length of incubation with sample reagent and finally
effects of long-term freezing on detection.