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Schiller, J., Müller, K., Süss, R., Arnhold, J., Gey, C., Herrmann, A., Lessig, J., Arnold, K. and Müller, P. (2003) Analysis of the Lipid Composition of Bull Spermatozoa by MALDI-TOF Mass Spectrometry—A Cautionary Note. Chemistry and Physics of Lipids, 126, 85-94.
https://doi.org/10.1016/S0009-3084(03)00097-5

has been cited by the following article:

• TITLE: A Simple Test of Seminal Fluid Chemistry and Its Potential Impact on Cryopreservation

  AUTHORS: Glenn Yeomans, Lindsay Penrose, Sam Prien

  KEYWORDS: Sperm, Cryopreservation, Customization, Technique

  JOURNAL NAME: Open Journal of Veterinary Medicine, Vol.13 No.9, September 14, 2023

  ABSTRACT: Cryopreservation is currently the only effective tool for long-term storage of semen in most species. However, it is well-recognized that, even in species that freeze well, some individuals resist cryopreservation. Work from this laboratory has demonstrated a relationship between maternal lipid content and the chemical constitution of the embryos they produce. The objective of the present study was to determine if a similar relationship might exist in paternal body chemistry and the animal’s semen sample and if such a difference could be determined with a simple weight test. Semen samples were obtained from cattle with known differences in body composition. The samples first underwent semen analysis and were then prepared as either cell-free (CF) or neat specimens (NS). Known volumes of each sample were weighed, and the remainder of the samples was analyzed for lipids, total proteins, and total carbohydrates using a series of spectrophotometric assays and blood chemistry techniques. As expected, weight differences were seen in the CF vs NS preparations of individual semen samples (p