TITLE:
A Simple Test of Seminal Fluid Chemistry and Its Potential Impact on Cryopreservation
AUTHORS:
Glenn Yeomans, Lindsay Penrose, Sam Prien
KEYWORDS:
Sperm, Cryopreservation, Customization, Technique
JOURNAL NAME:
Open Journal of Veterinary Medicine,
Vol.13 No.9,
September
14,
2023
ABSTRACT: Cryopreservation is currently the only effective tool for long-term storage of semen in most species. However, it is well-recognized that, even in species that freeze well, some individuals resist cryopreservation. Work from this laboratory has demonstrated a relationship between maternal lipid content and the chemical constitution of the embryos they produce. The objective of the present study was to determine if a similar relationship might exist in paternal body chemistry and the animal’s semen sample and if such a difference could be determined with a simple weight test. Semen samples were obtained from cattle with known differences in body composition. The samples first underwent semen analysis and were then prepared as either cell-free (CF) or neat specimens (NS). Known volumes of each sample were weighed, and the remainder of the samples was analyzed for lipids, total proteins, and total carbohydrates using a series of spectrophotometric assays and blood chemistry techniques. As expected, weight differences were seen in the CF vs NS preparations of individual semen samples (p