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Weerdenburg, E., Davies, T., Morrow, B., Zomer, A.L., Hermans, P., Go, O., Spiessens, B., van den Hoven, T., van Geet, G., Aitabi, M., DebRoy, C., Dudley, E.G., Bonten, M., Poolman, J. and Geurtsen, J. (2022) Global Distribution of O Serotypes and Antibiotic Resistance in Extraintestinal Pathogenic Escherichia coli Collected from the Blood of Patients with Bacteremia across Multiple Surveillance Studies. Clinical Infectious Diseases, 76, e1236-e1243. https://doi.org/10.1093/cid/ciac421

has been cited by the following article:

• TITLE: Detection of blaVIM Gene in β-Lactam Resistant E. coli Isolated from Clinical Samples in Ouagadougou, Burkina Faso

  AUTHORS: Abdoul Karim Ouattara, Blandine Ouédraogo, Rahimatou Yasmine Wendkuni Tiemtoré, Amana Mètuor Dabiré, Serge Sougué, Jacques Simporé

  KEYWORDS: E. coli, blaVIM, Cephalosporins, Multidrug Resistance, Burkina Faso

  JOURNAL NAME: Open Access Library Journal, Vol.10 No.8, August 23, 2023

  ABSTRACT: Escherichia coli, a Gram-negative bacterium, is commonly associated community and hospital-acquired infections, with significant antibiotic resistance implications for public health. This study aimed to detect the blaVIM gene in E. coli strains isolated from the Centre Hospitalier Universitaire Pédiatrique Charles De Gaulle (CHUP-CDG) in Ouagadougou, Burkina Faso. E. coli strains were isolated from various biological samples (urine, pus, blood, stool, and cerebrospinal fluid) from 2009 to 2013 at CHUP-CDG. Antibiotic susceptibility testing for cefotaxime, ceftazidime, and imipenem was performed using the disc diffusion method on Mueller-Hinton agar. Classical PCR was used to identify the blaVIM gene. The susceptibility test showed high resistance of strains to third generation cephalosporins. The resistance rate was 82.86% (29/35), 80.00% (28/35) and 11.42% (4/35) for cefotaxime, ceftriaxone, and imipenem, respectively. Analysis of PCR products revealed that 11.42% (4/35) of strains harbored the blaVIM gene. Significantly, 75.00% (3/4) of strains with the blaVIM gene were isolated from urine samples. The present study demonstrated the presence of the blaVIM gene in E. coli resistant strains to β-lactams at CHUP-CDG. Our results suggest the presence of other resistance genes in view of the low rate of blaVIM found in resistant strains. Surveillance measures are necessary to prevent the spread of these resistant strains.