TITLE:
Temperature and Pressure Behaviours of Methanol, Acetonitrile/Water Mixtures on Chromatographic Systems
AUTHORS:
Talal Aburjai, Muhammed Alzweiri, Yusuf M. Al-Hiari
KEYWORDS:
Retention Time, Pressure, Temperature, Methanol and Acetonitrile
JOURNAL NAME:
American Journal of Analytical Chemistry,
Vol.2 No.8,
December
8,
2011
ABSTRACT: Temperature and pressure were shown to vary significantly with solvent mixing, showing maxima at differ-ent solvent ratios. Acetonitrile/water mixing resulted in temperature reduction of solutions whereas metha-nol/water mixing caused temperature increases. On the other hand, maximum recorded chromatographic pressure of acetonitrile:water mixtures occurred at a solvent ratio of 1:6 compared with methanol:water, which showed a maximum pressure at a solvent ratio of 1:1. These findings can be of use in stabilizing re-tention time shifts during HPLC-based studies associated with compound identification based on retention time such as analysis of complex mixtures.