TITLE:
Rapid and Sensitive Analysis of Eight Polyphenols in Tobacco by Rapid Resolution Liquid Chromatogarphy
AUTHORS:
Fuwei Xie, Ajuan Yu, Dengke Hou, Huimin Liu, Li Ding, Shusheng Zhang
KEYWORDS:
Solid Phase Extraction, RRLC, Tobacco, Polyphenols
JOURNAL NAME:
American Journal of Analytical Chemistry,
Vol.2 No.8,
December
8,
2011
ABSTRACT: A rapid resolution liquid chromatographic (RRLC) method for the determination of eight polyphenols in to-bacco was developed. Polyphenols were extracted from tobacco samples by methanol/ water in an ultrasonic bath, then subjected to clean up by solid phase extraction. The separation was performed on a 50 × 4.6 mm, 1.8 μm ZORBAX Eclipse XDB-C18 column. Compared with conventional HPLC method, the analysis time for eight polyphenols by RRLC method was reduced from 20 to 5 min without sacrificing resolution, and the sensitivity was improved. This method appears simple, accurate and precious. The relative standard devia-tions (RSD) of overall analysis procedure for eight tobacco polyphenols were less than 2% with the recover-ies ranging from 94% to 107%. This method could be applied to the rapid determination of major polyphe-nols in tobacco with satisfactory results.