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S. Wolff, “Ferrous Ion Oxidation in Presence of Ferric Ion Indicator Xylenol Orange for Measurement of Hy- droperoxides,” Methods in Enzymology, Vol. 233, 1994, pp. 182-189. doi:10.1016/S0076-6879(94)33021-2

has been cited by the following article:

• TITLE: Direct Colorimetric Detection of Hydrogen Peroxide Using 4-Nitrophenyl Boronic Acid or Its Pinacol Ester

  AUTHORS: Gregory Su, Yibin Wei, Maolin Guo

  KEYWORDS: Hydrogen Peroxide Detection, 4-Nitrophenyl Boronic Acid, 4-Nitrophenyl Boronic Acid Pinacol Ester, 4-Nitrophenol, Colorimetric Method

  JOURNAL NAME: American Journal of Analytical Chemistry, Vol.2 No.8, December 7, 2011

  ABSTRACT: A colorimetric method for the direct determination of hydrogen peroxide in aqueous solution is described. H2O2 stoichiometrically converts 4-nitrophenyl boronic acid or 4-nitrophenyl boronic acid pinacol ester into 4-nitrophenol, which can be quantified by measuring the absorption at 400 nm in neutral or basic media. The reactions proceed fast under basic conditions and complete in 2 minutes to at pH 11 and 80?C. The linear range for the colorimetric method extends beyond 1.0 to 40 µM H2O2, and the limit of detection is ~1.0 µM H2O2. This method offers a convenient and practical process for rapid determination of hydrogen peroxide in aqueous media. Compared to many other techniques in H2O2 detection, this process is a direct measurement of H2O2, and is relatively unaffected by the presence of various salts, metal ions and the chelator EDTA.