TITLE:
Plant Regeneration through Indirect Organogenesis in Two Cultivars of Pineapple (Ananas comosus L.)
AUTHORS:
Gilles H. T. Cacaï, Brunith A. M. Ahokpossi, Serge S. Houédjissin, Jerome Anani Houngue, Bienvenu T. Badou, Corneille Ahanhanzo
KEYWORDS:
Callus Induction, Plant Growth Regulators, Stomata Structure, Rhizogenesis, Pineapple
JOURNAL NAME:
Open Journal of Applied Sciences,
Vol.13 No.7,
July
25,
2023
ABSTRACT: Unavailability of performant planting material of pineapple constitutes a
major problem of its cultivation in Africa. For this purpose, indirect
organogenesis technique is used to evaluate the in vitro responses of
two cultivars of pineapple during the explant’s regeneration. Calli were
induced from crown leaf and plantlets leaf of “Smooth Cayenne” and “Sugarloaf
cultivars”. Murashige and Skoog medium with vitamins B5 supplemented with different
growth regulators combinations were used. BAP and/or 2,4-D have been added to
base medium for calli cells’ differentiation while BAP and GA3 have been added for plant elongation.
The results indicated that explants from regenerated plantlets leaves cultivated on MS supplemented with copper (II) sulphate 5-hydrate
concentrations incorporated had significant (p mg/l) had a highly significant influence with 8.8 differentiated Calli.
Also, MS medium supplemented with BAP (3 mg/l) + GA3 (2 mg/l) for the “smooth
Cayenne” had significantly influenced (p mg/l of
NAA + 0 mg/l IBA produced a high number of roots in Sugarloaf whereas the medium containing 1.5 mg/l
NAA + 0.5 mg/l (IBA) produced high number of roots in smooth Cayenne. We have
established an efficient and reproducible protocol for mass propagation and genetic transformation of pineapple though indirect
organogenesis. This protocol may be used in genetics engineering studies for
pineapple breeding.