TITLE:
Isolation and Identification Methods for Oral Klebsiella pneumoniae Involved in Onset of Inflammatory Bowel Disease
AUTHORS:
Osamu Tsuzukibashi, Akira Fukatsu, Hiroshi Yamamoto, Yuji Takahashi, Keisuke Usuda, Mana Fuchigami, Chiaki Komine, Satoshi Uchibori, Koji Umezawa, Sachiyo Hayashi, Takashi Asano, Masanobu Wakami, Hiroshi Murakami, Taira Kobayashi, Masahiko Fukumoto
KEYWORDS:
Klebsiella pneumoniae, Selective Medium, Oral Cavity, Multiplex PCR
JOURNAL NAME:
Open Journal of Stomatology,
Vol.13 No.7,
July
13,
2023
ABSTRACT: Purpose: Recently, it was reported that Klebsiella pneumoniae is related to the onset of inflammatory bowel disease including the Crohn disease. It was frequently reported that K. pneumoniae was detected in human oral cavities. Regrettably, it currently remains unclear whether K. pneumoniae is part of the normal oral flora. The aim of this study was to establish the isolation and identification methods for K. pneumoniae from human oral cavities, and investigate its transmission pattern. Methods: A selective medium, OKPSM, for the isolation of K. pneumoniae from oral cavities was developed in this study. Also, PCR primer for the identification and detection at subspecies level of K. pneumoniae was designed. Results: OKPSM and PCR method using the primers designed in this study were useful for the isolation and identification of K. pneumoniae from human oral cavities. K. pneumoniae subsp. pneumoniae was detected at 10.0% in 30 saliva samples. On the other hand, K. pneumoniae subsp. ozaenae and K. pneumoniae subsp. rhinoscleromatis were detected from no sample. Moreover, K. pneumoniae subsp. pneumoniae isolates from same subject at 0 month and after 3 months showed same genotypes on AP-PCR using OPA-07 primer. Conclusion: These results indicated that human oral cavities were not suitable for the habitat of K. pneumoniae.