TITLE:
Isolation and Progeny Transmission of Non-Temperature-Sensitive MS-H Vaccine Strains of Mycoplasma synoviae from Temperature-Sensitive MS-H-Vaccinated Laying Breeder Hens
AUTHORS:
Victor M. Petrone-Garcia, Raquel López-Arellano, Inkar Castellanos-Huerta, Saeed El-Ashram, Ebtesam Al-Olayan, Danielle Graham, Guillermo Tellez-Isaias
KEYWORDS:
Vertical Transmission, Thermosensitivity, Mycoplasma synoviae, RAPD, Vax-Safe®
JOURNAL NAME:
Food and Nutrition Sciences,
Vol.14 No.7,
July
12,
2023
ABSTRACT: This pilot study reports the vertical transmission and reverse
thermosensitivity of the MS-H vaccine strain of Mycoplasma synoviae (MS) by RAPD in commercial breeders and their
progeny. At two weeks of age, breeders were vaccinated with the ts+ MS-H strain. At 9 weeks of age, an outbreak of infectious synovitis (IS) was
detected in the progeny. Tracheal swab samples were collected from breeders at
24, 39, 48, and 70 weeks of age. At 9 weeks, pullets swab from the elbow joints
were collected. RAPD was performed on the isolates at 39.5°C, and the
same ts- MS-H strains were identified in the breeder hens and their progeny. Tracheal
swabs from breeder hens were negative to MS isolation at 37°C and 39.5°C
at 24- and 39-weeks. MS isolation was recovered from tracheal swabs from 9/10
and 10/10 breeders at 48- and 70- week.
At 9 weeks of age in the progeny, MS was isolated from tracheal swabs of 10/10
from non-IS pullets. MS was isolated from 9/10 joints samples. The isolates
from breeder hens and their progeny showed non-significant differences in five
antimycoplasmic MIC100 values; otherwise, enrofloxacin presented a significant
difference in MIC100 value (p -H strain. Consequently, it is crucial to contemplate the danger of
reversing pathogenicity and transmission to progeny when applying the MS-H
vaccine strain.