TITLE:
Validation of High Performance Liquid Chromatography with Fluorescence Detector Methods for Determination of Aflatoxins in Different Food and Animal Feed Samples
AUTHORS:
Salah Eldeen Abass Ali Ahmed, Abdalla Ahmed Elbashir
KEYWORDS:
Aflatoxins, HPLC-FLD, Peanut, Peanut Butter, Sorghum and Animal Feeds
JOURNAL NAME:
Journal of Analytical Sciences, Methods and Instrumentation,
Vol.13 No.1,
January
31,
2023
ABSTRACT: Method validation for quantitative analysis of aflatoxins, AFB1, AFB2,
AFG1 and AFG2 in sorghum, peanut butter, groundnut and animals feed is
presented. Aflatoxins were extracted with a mixture of methanol: acetonitrile:
water (60:30:10) and cleaned with Alfa test IAC
chromatography before analysis by High Performance Liquid Chromatography
coupled with fluorescence detection (HPLC-FLD) by adopting an isocratic
chromatographic system using a mobile phase consisting acetic acid:
acetonitrile: methanol (59:14:27), the separation of the four aflatoxins was
achieved in less than 15 minutes. Calibration curves were linear over the
concentration range from 2 - 18 ng/mL for AFB1 and AFG1, 0.4 - 3.6 ng/mL for AFB2 and AFG2, respectively. The LOD and LOQ in spiked
samples were found to be 0.02 and 0.05 μg/kg for both AFB1 and AFG1, 0.01 and
0.03 μg/kg for both AFB2 and AFG2. The mean recovery values were in range from
84.2% to 96.9% was obtained. Five samples were found to be contaminated with
aflatoxins and the total aflatoxins ranged from 0.02 to 3.26
μg/kg were obtained. Nineteen different samples were found to be contaminated
with aflatoxins; the total aflatoxins ranged from 0.27 to 10.48
μg/kg were obtained. The highest total aflatoxins value was obtained in animal
feeds.