TITLE:
Molecular Identification of Isolated Bacteria from Soils in Likouala Peat Bog Area, Republic of Congo
AUTHORS:
Gatse Elgie Viennechie, Mboukou Kimbatsa Irène Marie Cécile, Morabandza Cyr Jonas, Mfoutou Mampanguy Claujeans Chastel, Ifo Suspens Averti, Nguimbi Etienne
KEYWORDS:
Bacteria, Peat Bog Area, PCR, Sequencing, 16S rRNA
JOURNAL NAME:
Open Journal of Soil Science,
Vol.13 No.6,
June
29,
2023
ABSTRACT: This study aimed to the molecular identification of isolated bacteria from the soils of Likouala (Congo-Brazzaville) peat bog area. Counting and isolation were carried out on Mossel and TSB media enriched with petroleum and vegetable oil; the amplification and sequencing of 16S RNA genes by PCR and Artic Oxford Nanopore Technology. The results showed bacterial loads of (5.81 ± 1.08) × 104; (6.64 ± 1.94) × 104 et (8.56 ± 1.19) × 103 CFU/g on Mossel respectively for samples 1, 2 and 3 against (2.12 ± 4.1) × 108 et (8.15 ± 10.1) × 107 CFU/g respectively on TSB enriched with petroleum and vegetable oil exclusively with sample 2. The analysis of the 16S rRNA of the isolates gene made it possible, after PCR, agarose gel electrophoresis, sequencing and bioinformatics analysis, to identify eight (08) strains with similarities of 99 to 100% whose sequences genes placed in GenBank have made it possible to obtain accession numbers corresponding to: Bacillus thuringiensis strain (ON303633); Bacillus cereus strain (ON350770); Bacillus thuringiensis strain (ON350771); Bacillus thuringiensis strain (ON738723); Priestia megaterium strain (ON738719); Bacillus anthracis strain (ON738720); Bacillus subtilis (ON738721); Enterobacter sp (ON738722). The phylogenetic classification of strains was done and revealed two genuses which are Bacillus and Enterobacteriaceae.