TITLE:
Codiaeum Variegatum Hydro Alcoholic Leaf Extracts and Their Fractions Inhibit Pro-Inflammatory Mediators in Vitro
AUTHORS:
Sylvain Pechangou Nsangou, Charifa Ngbetkom Mandou, Carine Matsigang Fondjou, Vigny Sayal Ngohoba, Edie Brice Enang II, Ibrahim Njingou, Emmanuel Mfotie Njoya, Frederic Nico Njayou, Paul Moundipa Fewou
KEYWORDS:
C. variegatum, Anti-Inflammatory, 5-Lipoxygenase, NO, TNF-α
JOURNAL NAME:
Journal of Biosciences and Medicines,
Vol.11 No.5,
May
11,
2023
ABSTRACT: Codiaeum variegatum has been widely investigated for its biological proprieties ranging from the antiamoebic potential to the phytochemical analysis. The aim of the present study was to evaluate the anti-inflammatory potential of C. variegatum leaf extracts and fractions. A primary macrophage culture activated by Saccharomyces cereviseae (SC) was used to evaluate cell cytotoxicity and anti-inflammatory potential of the plant extracts and fractions. Macrophages were treated with different concentrations (0.1; 1; 10 and 100 μg/mL) of the extracts/fractions for the inhibition of 5-lipoxygenase activity, nitric oxide (NO) and Tumor Necrosis Factor Alpha (TNF-α) production. No significant difference was observed on cell viability in the presence of extracts and fractions at tested concentration during the incubation period. Extracts and fractions of C. variegatum inhibited the 5-lipoxygenase activity, NO and TNF-α production by viable primary mouse macrophages in a concentration-dependent manner. The fractionation process increased anti-inflammatory activity. Among fractions, HEF2, HEF3, HEF5, EEF1, EEF3 and EEF5 exhibited the best anti-inflammatory potential. C. variegatum extracts and fractions exhibited a greater anti-inflammatory potential throughout the inhibition of pro-inflammatory mediators such as NO, 5-Lox and TNF-α.