TITLE:
Genetic Diversity Analysis of Badnaviruses Infecting Banana in Burkina Faso
AUTHORS:
Bakary Ouattara, Drissa Sérémé, Léon Wêndé-M’Minèré Nitiema, Issa Wonni, Kadidia Koita
KEYWORDS:
Banana Streak Virus, Sugarcane Baciliform Virus, RT/RNase H, Polymerase Chain Reaction, Diversity
JOURNAL NAME:
Advances in Bioscience and Biotechnology,
Vol.14 No.3,
March
30,
2023
ABSTRACT: Badnaviruses are serious plant pararetroviruses affecting banana and causes
serious economic losses to banana production worldwide. This study aims to examine the variability of BSV and SCBV nature
infecting banana in Burkina Faso. Polymerase Chain Reaction (PCR) used
the Badna FP/RP specific primers for the RT/RNase H regions present in
badnaviruses. The PCR yielded about 579 bp amplicons from banana infected by
BSV and SCBV. The 38 BSV isolates recorded low nucleotide identity ranging from
58.9% - 98.1%. Based on percentage nucleotide
sequence identity and phylogenetic analyse, BSV isolates were categorized into four groups: 1, 2, 3 and 4. Group 4
shared 76.9% - 100% identity with BSOL
species. However, Groups 1 and 3 recorded
a low identity ranging, from 76.8% -
79.2%, 68.8% - 79.7% with BSCV, and 72.8% - 79.0% between Group 2 and
BSOLV. Groups 1, 2 and 3 were assigned to
a potentially new BSV species. The two SCBV isolates recorded a low nucleotide
identity of 68% among themselves indicating high diversity. In addition, SCBV_Cd and SCBV_CE showed high nucleotide
identity 91.3% and 58.7% with SCBV_C and SCBV, when they were compared
to all published SCBV genotypes. In
addition, phylogenetic analysis revealed the segregation of SCBV
isolates into two genotypes, SCBV_Cd in C and SCBV_CE segregated in a new genotype namely Z. Recombination analyses
showed weak signatures of recombination among some of the BSV and SCBV sequences.