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Zheng, Y., Liu, W., Weng, Y., Polle, E. and Konzak, C.F. (2003) Chapter 2. Production of Doubled Haploids in Wheat (Triticum aestivum L.) through Microspore Embryogenesis Triggered by Inducer Chemicals. In: Maluszynski, M., Kasha, K.J., Forster, B.P. and Szarejko, I., Eds., Doubled Haploids Production in Crop Plants, A Manual, Kluwer Academic Publisher, Dordrecht, 83-94.
https://doi.org/10.1007/978-94-017-1293-4_14

has been cited by the following article:

• TITLE: Differential Effects of Cold and Heat Shock on Embryogenic Induction and Green Plant Regeneration from Wheat (Triticum aestivum L.) Microspores

  AUTHORS: Ming Y. Zheng, Allyson Fournier, Yujia Weng

  KEYWORDS: Albinism, Microspore Embryogenesis, Embryoid, Stress Treatment, Wheat

  JOURNAL NAME: American Journal of Plant Sciences, Vol.14 No.3, March 29, 2023

  ABSTRACT: Albinism is a common problem encountered by researchers in anther/microspore cultures of cereal crops. The present study investigates the effects of temperature variations on embryogenesis of wheat (Triticum aestivum L.) microspores. Following a cold (4°C - 13°C) vs. heat (33°C) shock to wheat tillers, microspores were isolated and cultured in a liquid medium to obtain embryoids. Data on embryogenic microspore%, embryoid yield, plant regeneration% and green plant% were collected and analyzed. Cold pretreatment of 4°C or 10°C for a period of 6 or 10 days were more effective than other cold temperature regimes in inducing microspore embryogenesis. The heat shock of 33°C yielded the highest numbers of embryogenic microspores and embryoids. The albino-prone genotypes produced significantly higher green plant% following optimal cold shock, as compared to the standard 33°C heat shock. Results from present study suggest that cold shock may be a desirable alternative for germplasm that produce lower green plant% using heat shock. Lowered incubation temperature during embryoid development did not result in higher green plant.