TITLE:
Construction of Fluorescence Sensing Platform on the Basis of Molybdenum Disulfide Nanosheet for the Detection of AFB1
AUTHORS:
Xiaoqing Wen, Zichun Song, Jiuying Cui, Yan Li, Qianli Tang, Xianjiu Liao
KEYWORDS:
MoS2NS, Aptamer, Aflatoxin B1, Fluorescence Sensing Analysis, Food Monitoring
JOURNAL NAME:
Journal of Biosciences and Medicines,
Vol.11 No.2,
February
8,
2023
ABSTRACT: Purpose: Aflatoxin B1 is the most common mycotoxin in cereal crops; it is of stronger toxicity and has a carcinogenic effect. In recent years, a series of fluorescence sensors constructed on the basis of MoS2NS fluorescence quenching property have become a research hotspot. Therefore, we can construct a fast and simple analysis method with high specificity to detect AFB1 by utilizing MoS2NS, which can be effectively applied to food safety monitoring and clinical diagnosis. Method: In the current research, a fluorescence biosensor is developed on the basis of a new type of two-dimensional nano-material namely MoS2NS applied for the detection of AFB1. The fluorescence of Apt@AFB1 can be quickly quenched by MoS2NS through the fluorescence resonance energy transfer (FRET). When the target molecule AFB1 exists, after the specificity binding between AFB1 and aptamer, the Apt@AFB1 loses its single stranded structure and is away from MoS2NS, and the fluorescence of Apt®AFB1 cannot be quenched effectively. Such sensing signals can be used to achieve the sensitive detection of AFB1. Result: With this new method, under the optimized conditions, the AFB1 is analyzed in the MoS2NS/Apt®AFB1 sensing platform. Within the dynamic range of 0.2 - 25 ng/mL, the sensing platform expresses a good linear response to the level of AFB1 with the R2 = 0.9964 and LOD as 90 pg/mL. This method is applied to detect the actual serum samples and soybean milk with the recovery rate of 93.10% - 107.23% and 95.15% - 102.60% separately, and it can be used in the quantitative detection under the interference of other mycotoxins in a relatively accurate way. Conclusion: It is proved that this new detection method can be used as a potential biosensor platform for the detection of AFB1. This detection method features several advantages such as specificity, rapidness and low costs, which can meet the requirement of trace detection in clinical detection and food safety.