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Scholarworks@uark, S. and Aljewari, H. (2019) Production and Purification of Basic Fibroblast Growth Factor Production and Purification of Basic Fibroblast Growth Factor Fused to two Collagen Binding Domains Expressed in E. coli BL21 Fused to Two Collagen Binding Domains expressed in E. coli BL21 Using Flask and Fed-Batch Using Flask and Fed-Batch.
https://scholarworks.uark.edu/etd/3521

has been cited by the following article:

• TITLE: Improved Catalytic Reaction of Biotemplated Palladium Nanoparticles through Immobilized Metal Affinity Purification

  AUTHORS: Shadrach Ibinola, Hazim Aljewari, Imann Mosleh, Robert Beitle

  KEYWORDS: Protein Expression, Nanoparticle Synthesis, Turnover Frequency, Suzuki-Miyaura

  JOURNAL NAME: Advances in Nanoparticles, Vol.12 No.1, February 8, 2023

  ABSTRACT: To investigate the effect purification plays on nanoparticle (NP) synthesis and catalytic activity, three copies of Pd4 (TSNAVHPTLRHL) fused to the N-terminus of Green Fluorescent Protein (GFP) was produced recombinantly and its characteristics pre and post purification was assessed. An E. coli expression system was employed, and purification was performed with Immobilized Metal Affinity Column (IMAC). Transmission electron microscopy (TEM) was utilized to examine the morphology of NPs synthesized with an enriched protein sample and ImageJ was used to determine the average size to be 2.44 nm. The turnover frequency of fabricated NP from the purified protein was analyzed by a model Suzuki-Miyaura coupling reactions and determined to be 33,000 hr-1. This value is three times higher than the turnover frequency when crude lysate containing (Pd4)3-GFP was used during NP synthesis. This result shows that enrichment enhanced the catalytic activity of NP.