TITLE:
Can the Prediction of Intrauterine Insemination Results by Used Aniline Blue Stain (ABS) and Sperm Chromatin Dispersion (SCD) Levels?
AUTHORS:
Talal Z. Al-Darawsha, Nurten Dayioglu, Bushra R. Al-Azzawi, Tulay Irez
KEYWORDS:
Sperm Chromatin Dispersion, Aniline Blue Stain, Sperm DNA Fragmentation, Intrauterine Insemination
JOURNAL NAME:
Advances in Reproductive Sciences,
Vol.11 No.1,
January
31,
2023
ABSTRACT: Introduction: This study aimed to perform routine seminal fluid analysis, sperm DNA fragmentation, and sperm function tests at the chromatin maturation level and evaluate pregnancy in the patients passing intrauterine insemination before starting Intrauterine Insemination (IUI) method. Materials and Methods: In this prospective study, 111 couples who underwent Intrauterine Insemination (IUI) in unexplained infertility patients were admittedto Al-Farah IVF and assisted reproductive center in Baghdad, Iraq between November 2020 and February 2021 were evaluated. Semen fluid analysis was performed based on (WHO 4th) guiding rules. In addition, Sperm ChromatinDispersion (halo test) and sperm maturation were performed with AnilineBlue Stain (ABS). Results: Sperm Chromatin Dispersion (SCD) groups werecompared in terms of pregnancy outcome; the positive pregnancy rate was found to be above in the normal SCD groups (p = 0.0005). In addition, Aniline BlueStain (ABS) groups were compared in the terms of pregnancy outcome; the positive pregnancy rate was found to be higher in the normal ABS group (p = 0.017). Conclusion: Our study showed that the use of DNA fragmentation (SCD)and sperm maturation tests (ABS) together with routine semen analysis in intrauterine insemination cases will make a significantcontribution to the prediction of Intrauterine Insemination (IUI) increased results. So, these results indicate a defect in the effect of DNA fragmentation on the outcome of intrauterine insemination.