TITLE:
Tumor Necrosis Factor-Alpha (TNF)-308G/A and Interleukin 8(IL-8)-251C/T Polymorphisms in Pulmonary Tuberculosis Patients from Congo
AUTHORS:
Faust René Okamba, Prudence Spinelie Koumba Pambou, Mandingha Kosso Etoka-Beka, Brave Nzoussi, Regis Gothard Bopaka, Cyr Jonas Morabandza, Gabriel Ahombo
KEYWORDS:
Pulmonary Tuberculosis, Cytokine Polymorphism, Tumor Necrosis Factor-Alpha, Interleukin-8, PCR-RFLP
JOURNAL NAME:
Open Journal of Immunology,
Vol.13 No.1,
January
18,
2023
ABSTRACT: Background: Tuberculosis (TB) is one of the world’s
deadliest infectious diseases. Tumor necrosis factor-Alpha (TNF-α) and Interleukin 8 (IL-8) are involved
in the pathogenesis of pulmonary TB (PTB). However, the contribution of
polymorphisms of these cytokines to PTB susceptibility needed more
investigation across geographic regions and ethnic groups. Purpose: The
aim of this study was to investigate the association of the TNF-α-308 G/A and IL-8-251T/A polymorphisms
with PTB risk in the Congolese population. Methods: This case-control
study included 150 PTB patients and 160 control subjects. Blood samples were collected
from all participants and were used for the TNF-α-308 G/A and IL-8-251T/A genotyping by polymerase chain
reaction-restriction fragment length polymorphism (PCR-RFLP) technique. Odds
ratios (OR) were calculated to estimate the potential polymorphism
associations. A P level of Results: A significant difference was found between PTB patients and controls regarding
the TNF-α-308AA genotype (P = 0.035) distribution. Moreover, this
genotype was associated with risk to TB (OR = 7.19, 95% CI = 0.85 - 60.65, P = 0.035). The A allele was significantly more frequent in PTB patients than
in controls, and was associated with risk to PTB (OR = 1.68, 95% CI = 1.05 - 2.68, P = 0.014). Regarding the IL-8-251T/A gene, TA and AA genotypes were
significantly more frequent in PTB patients compared to controls, and were
associated with increased risk to PTB (OR = 2.64, 95% CI = 0.97 - 7.18, P = 0.031 and OR = 3.0, 95% CI = 1.13 - 7.98, P = 0.014, respectively). However, the IL-8-251
A allele was not associated to PTB susceptibility (OR = 0.27, 95% CI = 0.15 - 0.44). Conclusion: TNF-α-308G/A
and IL-8-251T/A polymorphisms may be associated to PTB susceptibility in the
Congolese population, and the AA genotype of both cytokines could be a risk
factor.