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Dawson, I.K., McMullin, S., Kindt, R., Muchugi, A., Hendre, P., Lilleso, J.P.B. and Jamnadass, R. (2019) Delivering Perennial New and Orphan Crops for Resilient and Nutritious Farming Systems. In: Rosenstock, T.S., Girvetz, E. and Nowak, A., Eds., The Climate-Smart Agriculture Papers, Springer, Cham, 113-125.
https://doi.org/10.1007/978-3-319-92798-5_10

has been cited by the following article:

• TITLE: Insight into Genetic Diversity of Cultivated Lima Bean (Phaseolus lunatus L.) in Benin

  AUTHORS: Rose Fernande Fagbédji, Lambert Gustave Djedatin, Chimène Nanoukon, Geofroy Kinhoegbe, Amed Havivi, Hounnankpon Yédomonhan, Clément Agbangla

  KEYWORDS: Phaseolus lunatus L., Benin, Genetic Diversity, SSR Markets, Conservation

  JOURNAL NAME: American Journal of Molecular Biology, Vol.13 No.1, December 30, 2022

  ABSTRACT: Lima bean is a tropical and subtropical legume from the genus Phaseolus which is cultivated for its importance in food and in medicine, but which remains a Neglected and Underutilized Crop in Benin. Understanding the genetic diversity of a species’ genetic resources is useful for the establishment of appropriate conservation strategies and breeding programs and for sustainable use. We use 6 out of ten SSR markers to analyze the diversity and population structure of 28 Lima bean landraces collected in Benin. A total of 28 alleles with an average of 4.16 alleles per SSR were amplified. The Polymorphic Information Content value ranged from 0.079 to 0.680 with an average of 0.408. The analysis of population structure revealed three subpopulations. PCoA revealed three well-separated clusters among the analyzed accessions in accordance with the population structure results and the clustering based on the Neighbor-Joining tree. AMOVA showed highly significant (p = 0.001) diversity among and within populations. Hence, 32% of the genetic variation was distributed among the population and 68% was distributed within populations. A high PhiP value (0.321) was found between the three sub-subpopulations indicating a high genetic differentiation between these sub-subpopulations. By exhibiting the highest average number of alleles, Shannon-Weaver information and Shannon-Weaver diversity indices, and the highest mean number of private alleles, subpopulation 1 is the main gene pool of the analyzed collection. The present study is an important starting point for the establishment of appropriate conservation strategies and breeding programs for Lima bean genetic resources.