TITLE:
Ionic Liquid Efficiency on Wood Dissolution and Polysaccharide Identification
AUTHORS:
Idelette Plazanet, Christelle Gady, Sabine Lhernould, Rachida Zerrouki, Daniel Montplaisir, Guy Costa
KEYWORDS:
Plant Cell Wall, Antibody, Immunolabeling, Imidazolium Based IL, DBU Based IL
JOURNAL NAME:
Advances in Biological Chemistry,
Vol.12 No.6,
December
29,
2022
ABSTRACT: The wood polysaccharide composition, a new analytical method, based on ionic liquid dissolution of low amount of biomass coupled with an ELISA essay of polysaccharides. In the present work, we synthesized and tested several imidazolium and 1,8-diazabicyclo[5.4.0]undec-7-ene (DBU) based ILs for their ability to solubilize Douglas-fir wood while preserving the wall polymer integrity. The couple times-temperatures have been essayed for wood dissolution. Then their efficiency for wood biomass dissolution was compared to the impact of IL on storing and/or destroy polysaccharides. Thanks to the ELISA technique with a set of mAbs against epitopes of the main hemicellulose, pectin, and protein families of cell wall components. Wood destructuration at 80˚C with the 1-ethyl-3-methylimidazolium bromide represents a good compromise of wood dissolution efficiency and low polysaccharide destruction.