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Gilchrist, C.A., Cotton, J.A., Burkey, C., Arju, T., Gilmartin, A., Lin, Y., Ahmed, E., Steiner, K., Alam, M., Ahmed, S., et al. (2018) Genetic Diversity of Cryptosporidium hominis in a Bangladeshi Community as Revealed by Whole Genome Sequencing. The Journal of Infectious Diseases, 218, 259-264.
https://doi.org/10.1093/infdis/jiy121

has been cited by the following article:

• TITLE: Comparative Performance of Microscopy and Nested PCR for the Detection of Cryptosporidium Species in Patients Living with HIV/AIDS in Abidjan (Côte d’Ivoire)

  AUTHORS: N’Docho Assi Fiacre-Tanguy, Gonédélé Bi Sery Ernest, Tuo Karim, Bialé Marina Danielle Datcho-Gouenet Ayaud, N’Guessan Tiacoh Landry, Koffi Attoungbré Clément, Koffi David, Ako Ako Aristide Bérenger, Vanga Bosson Abo Henriette, Touré Offianan André

  KEYWORDS: Cryptosporidium, PCR, Microscopy, PLHIV, Côte D’Ivoire

  JOURNAL NAME: American Journal of Molecular Biology, Vol.13 No.1, December 27, 2022

  ABSTRACT: Cryptosporidium spp. infection is one of the causes of diarrhea in people living with HIV/AIDS. The objective of this study is to compare the sensitivity of microscopy and molecular biology to determine the prevalence of Cryptosporidium spp. in Patients Living With HIV (PLWH). This is a descriptive cross-sectional study conducted in three care centers for people living with HIV/AIDS in Abidjan. It took place from November 2018 to March 2020. Sociodemographic data were obtained via a questionnaire. Stool and blood samples were collected and analyzed for microscopy and Nested PCR detection of Cryptosporidium spp. Blood samples were analyzed for CD4+ count. A total of 363 stool samples were collected from the three sites. Individuals aged 40 - 50 years (36.52%) were most likely to participate in the study. HIV Type 1 accounted for 86.22% of the study population. The samples collected consisted of 47.65% diarrheal stool. Microscopic examination of the stool yielded a prevalence of 3.86% for Cryptosporidium spp. while the prevalence was 3.96% with molecular identification. No statistically significant difference was observed between these two prevalences (χ2 = 0.26; p = 0.609). CD4+ count was the factor associated with Cryptosporidium spp. infection for both microscopy (OR = 0.887, p = 0.001) and PCR (OR = 0.896, p = 0.001). This study demonstrated that Nested PCR improves the detection of Cryptosporidium spp. in patient diagnosis.