TITLE:
Superoxide Anion Release by Human Blood Phagocytes Can Increase the Microbicidal Activity Induced by a New Microemulsioned System Containing Cotton Oil
AUTHORS:
Luana Cristina da Silva Ramos, André Henrique Furtado Torres, Letícia Damas Leão Dalcin, Katleyn Polizeli Galvão Silva, Luana Gabriela Silva de Freitas, Mahmi Fujimori, Danny Laura Gomes Fagundes, Eduardo Luzia França, Adenilda Cristina Honorio França
KEYWORDS:
Cotton Oil, Microemulsion, Phagocytosis, Infection, Human Phagocytes
JOURNAL NAME:
Advances in Biological Chemistry,
Vol.12 No.6,
December
27,
2022
ABSTRACT: The study aimed to develop and characterize a microemulsified system based on cotton oil and verify its effect on superoxide release anion and microbicidal activity by human peripheral blood cells. Microemulsions were formulated using distilled water, degummed cotton oil, Span 80 (SP), Tween 80 (TW), and 1-butanol (BT). The pseudo-ternary diagram delimited ME regions, and the points were pre-selected. The physical-chemical and rheological characterization of the microemulsions was carried out. The ME activity on the interactions between leukocytes and bacteria was analyzed by superoxide release, phagocytosis, and microbicidal activity. The developed formulation was classified as Oil/Water, with an average pH of 5.76, and the viscosity showed resistance to temperature changes. The rheological model of the Power Law classified the microemulsion as a non-Newtonian fluid with pseudoplastic characteristics. The cell viability of cotton oil microemulsion was greater than 90%. There was an increase in the superoxide release by MN phagocytes when treated with cotton oil microemulsion. The cotton oil microemulsion increased phagocytosis and microbicidal activity. The present study suggests that cotton oil is an alternative biomaterial for therapeutic applications, especially in treating infections.