TITLE:
Effect of Kombucha and Its Non-Polar Components on Morphological Aspects of the Pancreas of Diabetic Rats with Streptozotocin
AUTHORS:
Guillermo Nolasco-Rodríguez, Rubén González-Valadez, Lucia García-Delgado, Esther Albarrán-Rodríguez, José Ricardo Cuellar-Pérez, Jacinto Bañuelos-Pineda, Manuel Rosales-Cortés
KEYWORDS:
Diabetes, Kombucha, Rats, Streptozotocin, Non-Polar Components of Kombucha
JOURNAL NAME:
Open Journal of Veterinary Medicine,
Vol.12 No.12,
December
14,
2022
ABSTRACT: Objective: to evaluate the protective effect of kombucha and its components not polar, in pancreas of diabetic rats treated with streptozotocin. Material and methods: ninety-six male Wistar rats of 120 to 170 g of PC were used; four groups of 24 animals were formed: control (GC), control treatment (GCTx), kombucha treatment (GTxK) and treatment with non-polar components of kombucha (GTxCNP). Diabetes was induced in the last three groups with an intraperitoneal injection of streptozotocin at a dose of 65 mg/kg BW. At the same time, treatment was started in the different groups; GC and GCTx groups were administered, orally, 1 ml of sterile saline solution; to the GTxK group 324 mg/kg of kombucha; and to GTxCNP 0.006 mg/kg of the non-polar components. For the histopathological study, pancreas samples were obtained on days 1, 7, 14 and 21 and fixed in formalin. They were processed with the histological technique, 5-micron-thick sections were made and stained with the hematoxylin-eosin technique. Finally, the number of islets of Langerhans per field observed with the 10x objective was quantified. The results were subjected to a one-way analysis of variance and Tukey’s test (p Results: Significant changes in the number of islets were observed in histological sections of the pancreas. The groups treated with streptozotocin showed a significant decrease in the number of islets 7 days after starting treatment; Likewise, their number was lower in the GCTx group compared to the GTxK and GTxCNP groups (p ≤ 0.05). At 14 and 21 days, the lowest number of islets was maintained, although only in the GCTx and GTxK groups (p ≤ 0.05). Streptozotocin is a drug that can be used in rats to induce an experimental model of diabetes with an effect that can last up to 21 days. Conclusions: This fermented beverage and its non-polar components can be a complementary treatment alternative for diabetes mellitus, since it prevents further damage to the β cells of the pancreas and the Islets of Langerhans. Specific additional studies on the main, non-polar components of kombucha are suggested to know the mechanism of protection on pancreatic islets.