TITLE:
In-Silico and Biological Analysis of B-Cell Lymphoma-2 Gene and Genetic Mutation as Diagnostic Marker in Childhood Sudanese Acute Lymphoblastic Leukemia Patients, Gezira State, Sudan (2018)
AUTHORS:
Samar Abdalaziz, Asad Adam Abbas, Soad Fadal Allah, Yousif Abdelhammed, Ozaz Y. M. Ahmed, Hisham N. Altayb
KEYWORDS:
ALL, BCL2, DNA
JOURNAL NAME:
Open Access Library Journal,
Vol.9 No.11,
November
28,
2022
ABSTRACT: Objective: To analyze the BCL2 genetic as diagnostic marker among acute lymphoblastic leukemia in childhood Sudanese patients, to detect genetic polymorphism in BCL2 in childhood acute lymphoblastic leukemia patients using Polymerase chain reaction (PCR) and DNA sequencing technique to confirm the harmfulness of the detected mutation using in silico analysis. Material and Methods: Venous blood was drained by means of clean venipuncture into a labeled 5 ml K-EDTA tubes. Subsequent mixing of blood with anticoagulant was followed and the tubes were stored at -20°C, 40 blood samples were carried out using Sysmex XP-300 automated hematology analyzer, DNA extraction using innuPREP blood DNA mini extraction kit, quality of the purified DNA was evaluated by electrophoresis in 0.25 g agarose gel & DNA amplification using polymerase chain reaction. Results: In this study, sequencing showed that harmful mutation of a homozygous AA allele in one case of pre B-ALL and a heterozygous mutation AC allele in one control, BCL2 promoter region polymorphism is more reliable gene promoter polymorphism in ALL.