TITLE:
Isolation and Expression Analysis of Meristem Gene CLV2 and Carpel Generation Gene (CRC) from Multi-Carpel Structure of Thermopsis turcica
AUTHORS:
Ferruh Aşçi, Arzu Unal, Sultan Demir Şimşek
KEYWORDS:
Thermopsis turcica, CLV, CRC, cDNA, PCR, RACE, RT-qPCR, In Situ
JOURNAL NAME:
Advances in Bioscience and Biotechnology,
Vol.13 No.11,
November
22,
2022
ABSTRACT: Thermopsis turcica is an endemic species of wild legume in Türkiye. The plant produces
flower pistils with unusual morphological properties. The aim of this study was to
determine the factors controlling the interesting feature of this plant.
Particularly the controlling features of the flower meristem and the pistils that mutations in two genes and helps
occurring the number of flower organs and can cause abnormalities, This
study has attempted to explore the controlling feature of Thermopsis turcica,
the young bud Arabidopsis CLV2, and CRC gene homologs were isolated and
analyzed in different tissues. First, with total RNA isolated from the first
thread degenerated primers synthesized cDNA expressions, second with a partial length cDNA cloning process made and the
replicated set of analyses. The full-length
cDNA of genes related to RACE analysis was
carried out with the sequencing process. The real-time PCR method was applied for gene
functional analyses with buds, sepals, petals and stamen and relative
expression analysis of genes in the tissue of pistils. Full-length cDNA
sequence for the gene CLV2 2557 bp,
the encoded protein 716 amino acids, CRC 901 for full-length cDNA sequence of the gene—bp, the
encoded protein 174 identified as amino acids. Alignment and phylogenetic analyses of CLV2 and CRC genes were found
to be similar to other types of legumes in homologous sets. Real-time (RT-qPCR) PCR results relative to study courses
with genes.