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Valko, M., Leibfritz, D., Moncol, J., et al. (2007) Free radicals and antioxidants in normal physiological functions and human disease. International Journal of Biochemistry & Cell Biology, 39(1), 44-84.

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• TITLE: DNA damage and cell death assessment in patients with severe multiple trauma using comet assay

  AUTHORS: Aliy K. Zhanataev, Victor V. Moroz, Andrey D. Durnev, Maria Yu. Muravyeva, Vasiliy I. Reshetnyak

  KEYWORDS: Apoptosis; Necrosis; Multiple Trauma; DNA-Comet Assay; 8-Oxoguanine;Total Antioxidant Status

  JOURNAL NAME: Health, Vol.2 No.5, May 27, 2010

  ABSTRACT: Purpose: To determine the DNA strand breaks, oxidative DNA damage and cell death in blood and plasma total antioxidant status (TAOS) in 22 patients with severe multiple trauma. Materials and methods: The DNA comet assay was used to measure DNA strand breakage, 8-oxoguanine levels and apoptotic and necrotic nuclei in after admission (day 0) and on days 3, 5, 7 and 15. TAOS was determined by colorimetric method. Results: Trauma patients had high DNA damage at admission (p