TITLE:
Incidence of Sickle Cell Disease and Other Hemoglobinopathies in Burkina Faso: Results of a Five-Year Systematic Neonatal Screening (2015-2019) in Four Urban Hospitals
AUTHORS:
Salam Sawadogo, Koumpingnin Nebie, Donatien Kima, Hélène Traore Savadogo, Jean De Dieu Sanou, Dieudonné Ouedraogo, Paul Ouedraogo, Hyacinthe Zamane, Abdoulaye Ndiaye, Eléonore Kafando
KEYWORDS:
Abnormal Hemoglobin, β-Globin Gene, Newborn Screening, Isoelectric Focusing
JOURNAL NAME:
Open Journal of Blood Diseases,
Vol.12 No.4,
November
16,
2022
ABSTRACT: Hemoglobinopathies,
mainly Sickle cell disease (SCD), are the most common monogenic disorders in
Africa. In Burkina Faso, data on these diseases are scarce, mainly hospital-based in Ouagadougou and its
surroundings. In order to assess the incidence and allelic frequencies of the
main hemoglobinopathies in newborns in Burkina Faso, we conducted a
cross-sectional study from 2015 to 2019 in four hospitals. The study included
babies of both sexes, regardless of ethnic group and parents’ hemoglobin
status. It was a newborn screening and hemoglobin variants were detected using
isoelectric focusing on cord blood samples and confirmed using hemoglobin
electrophoresis by high-performance liquid chromatography. The proportions
and cumulative incidences of the different hemoglobinopathies were computed.
Hardy-Weinberg equilibrium law was applied to calculate
genotypic and allelic frequencies. The significant level was p ere from Bobo-Dioulasso. Abnormal
hemoglobin was found in 27.1%, representing a cumulative incidence of 1:4
newborns. The incidence of SCD was 1.9% (1:53 newborns) with 27.9% of
homozygous SS. Homozygous CC and compound heterozygous Cβ-Thalassemia accounted for 1.1%. SCD cases were 1.51 times higher
in Bobo-Dioulasso (OR = 1.51;
95% CI [1.09 - 2.10]: p = 0.013). The
observed genotype frequencies were significantly different from the expected
ones (p 0.001). The βS and βC alleles represented 5.1 and 9.9%, respectively. This study showed
a high incidence of hemoglobinopathies. Such results raise the question of
control strategies for these hemoglobinopathies in our country.