TITLE:
Streptococcal Pyrogenic Exotoxin Genes SpeA and SpeB in Isolates of Streptococcus pyogenes from Children with Pharyngitis, Gezira State, Sudan
AUTHORS:
Minas Mohamed Balla, Adil Mergani, Mohamed Elamin A. M. E. Medani, Adam Dawoud Abakar, Ameer Mohamed Dafalla
KEYWORDS:
Streptococcus pyogenes, Streptococcal Pyrogenic Exotoxin Genes, Sudan, SpeA, SpeB
JOURNAL NAME:
American Journal of Molecular Biology,
Vol.12 No.4,
October
20,
2022
ABSTRACT: Background: Streptococcus pyogenes (group A streptococcus, GAS) is an important human
bacterial pathogen. This organism possesses many virulence factors,
Streptococcal pyrogenic exotoxinone of these. Aim: Detection of
Streptococcal pyrogenic exotoxin SpeA and SpeB in isolated Streptococcus
pyogenes. Methods: Tow hundred throat swab samples were collected
from children with pharyngitis referred to Pediatric Teaching hospital and ENT
hospital Wad medani, Sudan, from January to November 2021. The questionnaire was filled out to collect clinical and demographic data. Throat
swabs were collected and processed with the standard microbiological procedure
to isolate Streptococcus pyogenes. Antimicrobial susceptibility testing
was done on all
GAS isolates using the Kirby Bauer disk diffusion method according to clinical
laboratory standard institute (CLSI) guidelines. Detection of Spy 1258 gene and
Streptococcal pyrogenic exotoxins SpeA and SpeB were done by using Multiplex
PCR. Results: Amongst the Tow hundred collected samples fifty-one isolates (25.5%) were identified as S. pyogenes.
Antibiotic susceptibility testing showed that all the GAS isolates were
sensitive to Azithromycin and Penicillin. Sensitivity to Erythromycin,
Gentamicin, Clarithromycin, Amoxicillin and Cephalexin were 88.2%, 86.3%, 45.1%, 41.2%, 13.7%,
respectively. SpeA was detected in 17 (33.3%) and SpeB in 48 (94.1%). Conclusion: Streptococcal pyrogenic exotoxin genes SpeA and SpeB were detected in 17 (33.3%)
and 48 (94.1%) respectively of Streptococcus pyogenes isolates.