TITLE:
Development and Quantification of Fungal Biofilm in Acrylic Resins of Dental Prostheses Pretreated with Rosmarinnus officinalis
AUTHORS:
Maria Elvira S. Cruzeiro, Rogério Freitag, Daiane E. Blank, Luciana Pinto, Mário Carlos A. Meireles, Marlete Brum Cleff
KEYWORDS:
Biofilm, C. albicans, Plant Extracts, Rosmarinus officinalis
JOURNAL NAME:
Open Access Library Journal,
Vol.9 No.10,
October
11,
2022
ABSTRACT: Candida albicans is the main agent of oral candidiasis being classified as an opportunistic non-contagious yeast, also the main species involved in the development of this disease. Dentures are commonly biofilm deposits, especially the fungal biofilm that became the target of several studies, being the treatment of stomatitis with classic antifungal agents well established. However, resistance by individuals has been described. The objective of this study was to quantify the biofilm formation of Candida albicans in denture material pretreated with extracts of Rosmarinus officinalis. For the preparation of the specimens, microwave cured acrylic resin was used, being polished and sterilized with ethylene oxide, and divided into six groups according to the treatment used: nystatin, lotion of 24% of R. officinalis, essential oil of R. officinalis, hydroalcoholic extract of R. officinalis, carbopol and controls (untreated). The specimens were immersed in treatment for 14 days; then, the biofilm was induced and quantified. Data were statistically analyzed by ANOVA followed by Tukey test (α = 0.05). The lotion R. officinalis at 24% was the most effective in preventing biofilm formation, reducing it by 96.6% compared to control, followed by the essential oil in carbopol with 83%, being those results not statistically different compared to nystatin (68.3%). The hydroalcoholic extract did not prevent biofilm formation. We conclude that the lotion at 24% and the essential oil when applied to the acrylic surface, are capable of inhibiting fungal biofilm formation.