TITLE:
The Protective Effects of Preventive Atomisation Inhalation of Edaravone on the Lung Tissues of Rats with Smoke Inhalation Injury
AUTHORS:
Ruluo Qian, Changshuan Xiao, Yaping Liu, Jingzhe Yang
KEYWORDS:
Edaravone, Rats, Prevention, Smoke Inhalation Injury
JOURNAL NAME:
American Journal of Molecular Biology,
Vol.12 No.4,
September
30,
2022
ABSTRACT: Objective: To investigate the
protective effects of the atomisation inhalation of edaravone on the lung
tissues of rats with smoke inhalation injury. Methods: Forty male
Sprague-Dawley (SD) rats were randomly divided into four groups of ten rats
each: normal control group (group A), normal saline atomisation group
(group B), edaravone aerosol group (group C) and edaravone atomisation
prevention group (group D). Barring group A, the groups were used to create a
model of severe smoke inhalation injury. However, before developing the model,
group D rats were made to inhale edaravone (3.6 mg/mL) for 10 min. Six hours
following smoke inhalation injury, abdominal artery blood samples were
centrifuged, the lung tissue homogenate was prepared and carotid artery blood
samples were used for blood gas analysis and oxygenation index (PaO2/FiO2)
calculation. The levels of tumour necrosis factor alpha (TNF-α), interleukin (IL) 6 and IL-10 in
serum and the levels of cysteine protease 3 (caspase-3), malondialdehyde (MDA),
myeloperoxidase (MPO) and superoxide dismutase (SOD) in lung tissues were
examined. The wet-dry ratio (W/D) and water content of the lung tissue were calculated,
and the TUNEL method was used to determine the rate of lung tissue apoptosis in
each group. Tissue specimens were obtained from the partial lung for
histopathological examination. Results: Compared with those in group A,
the water content of the lung tissue, the rate of lung tissue apoptosis, W/D
and the caspase-3, TNF-α, IL-6, IL-10,
MDA and MPO levels were significantly greater in other groups (P2/FiO2 were lower (P Compared with those in group B, the
levels of W/D, the water content of the lung tissue, the rate of lung tissue
apoptosis and the levels of caspase-3, TNF-α,
IL-6, MDA and MPO were significantly low (P and the
levels of IL-10, SOD and PaO2/FiO2 were significantly
high in groups C and D (P The expression of the
aforementioned factors was more evident in Group D (P Conclusions: The inhalation of edaravone can
reduce smoke inhalation-induced lung injury. This may be related to the
inhibition of apoptosis, the reduction of peroxidation injury and the
production/release of inflammatory mediators/free radicals. It exerts a
remarkable preventive effect.