TITLE:
Comparative Study on Semen Quality and Fertility of Red Chittagong Cattle, BCB1 and Munshiganj Bulls of Bangladesh
AUTHORS:
Sheikh Mohammad Jahangir Hossain, Md. Faizul Hossain Miraz, Shahrina Akter, Md. Younus Ali, Gautam Kumar Deb
KEYWORDS:
Native Bulls, CASA, Sperm Kinematics, Morphology and Fertility
JOURNAL NAME:
Advances in Bioscience and Biotechnology,
Vol.13 No.9,
September
29,
2022
ABSTRACT: The present study was conducted to analyze the fresh and post-thaw semen
quality and fertility from native bulls of Red Chittagong Cattle (RCC), BLRI
Cattle Breed 1 (BCB1), and Munshiganj Cattle of Bangladesh. One hundred and
seventy-two ejaculates were collected by artificial vagina set and semen
analysis was performed using Computer Assisted Sperm Analyzer (CASA) at
Bangladesh Livestock Research Institute. Commercial extender (AndroMed) was
used to dilute the fresh semen. After equilibration (4°C for 4 hr),
freezing was done using a programmable bio-freezer. Post-thawed semen was
evaluated for sperm motility and kinematics. Cryopreserved semen straws were
used for artificial insemination (AI) and determined the bull fertility based
on 60 days non-return rate. Motility of the sperm differs significantly (p 0.05) among the bulls but the
highest concentration was found in Munshiganj bull (1669.60 ± 192.07
million/ml) followed by RCC (1648.70 ± 91.07 million/ml) and BCB1 bull (1481.60
± 167.35 million/ml). Moreover, the highest bent tail (5.89 ± 0.75%), coiled
tail (1.01% ± 0.22%) and distal mid-piece reflex (2.26% ± 0.28%) were observed
in BCB1 followed by Munshiganj and RCC. Amplitude of lateral head displacement
(ALH) was recorded higher in post-thaw than in fresh semen. Kinematics
parameters of post-thaw semen decreased than fresh semen irrespective of
genotypes. More number of doses/ejaculates can be produced from Munshiganj bull
(394.34 ± 127.95) followed by RCC (349.01 ± 120.91) and BCB 1 bulls (331 ±
98.99). Fertility rate does not differ among the bulls (p > 0.05) but the
highest value was found for RCC (62.06% ± 1.94%) bulls. Therefore, it can be
concise that, the quality of Red Chittagong Cattle semen is better than BCB 1
and Munshigang bull.