TITLE:
Particle Size Effects on Antioxydant and Hepatoprotective Potential of Essential Oil from Eucalyptus camaldulensis Leaves against Carbon Tetrachloride-Induced Hepatotoxicity in Rats
AUTHORS:
Venant Donald Noumi, Markusse Deli, Richard Marcel Nguimbou, Elie Baudelaire, Sandrine Rup-Jacques, Dicko Amadou, Selestin Sokeng, Nicolas Yanou Njintang
KEYWORDS:
Eucalyptus camaldulensis, Powder Particle Size, Essential Oils, Hepatoprotection, Oxidative Stress
JOURNAL NAME:
Pharmacology & Pharmacy,
Vol.13 No.8,
August
5,
2022
ABSTRACT: Particle size fractionation by sieving is used to optimize antioxydant potential of natural substances. The aim of the present study was to evaluate particle size effects on antioxidant and hepatoprotective potential of Eucalyptus camaldulensis essential oils (EO) on CCl4-induced hepatic damage in Wistar rats. Animals were daily orally treated with the EOs extracted by hydrodistillation from powder sieved at four particle sizes (≥355, 200 - 355, 100 - 200, ≤100 μm) and those of the unsieved powder at dose of 50 mg/Kg for 7 days. Compounds that are evaluated for these activities are hydrocarbons and oxygenated terpenes that were identified and quantified by GC/MS. Activities of enzymes markers of hepatocellular damage in serum and antioxidant enzymes in the liver homogenates were measured. In this research, EOs significantly prevented the increase in serum ALT and AST (p 4 and significant increase level of plasma HDL-cholesterol. Also, significantly (p