TITLE:
In Vitro Screening of Endophytic Trichoderma sp. Isolated from Oil Palm in FGV Plantation against Ganoderma boninense
AUTHORS:
Elya Masya Mohd Fishal, Ili Bazilah Abd Razak, Nor Hidayah Bohari, Muhammad Ashraf Arif Mohd Nasir
KEYWORDS:
Endophytic Trichoderma, Ganoderma boninense, In Vitro, Oil Palm
JOURNAL NAME:
Advances in Microbiology,
Vol.12 No.7,
July
29,
2022
ABSTRACT: The potential of several endophytic Trichoderma isolates, isolated from healthy oil palm roots was assessed against Ganoderma boninense (PER71), the causal agent of Basal Stem Rot (BSR) disease through in vitro screening. In the dual culture assay, all endophytic Trichoderma isolates were found to be capable of inhibiting the growth of PER71 mycelium, by causing more than 70% inhibition of the pathogen’s radial growth (PIRG). Trichoderma asperellum M103 has caused the highest inhibition at 93.14%. The volatile compound produced by M103 also suppressed 65% of the pathogen’s radial growth. On the other hand, T. harzianum, M108 was found to have the ability to produce non-volatile compounds that is toxic and it has effectively inhibited 98.18% of the growth of PER71. Collectively, T. asperellum M103 and T. harzianum M108 have the potential to inhibit the growth of G. boninense PER71. These isolates were shown to be viable options for the future management of G. boninense in the oil palm field.