TITLE:
Antibiotic Resistance and Phynotypic Detection of AmpC Beta-Lactamase Producing Escherichia coli from Urine of Students Attending Fulafia Clinic
AUTHORS:
Usman S. Okposhi, Kabiru A. Shuaibu, Chuku Aleruchi, Fatima A. Yusuf, Naja’atu S. Hadi
KEYWORDS:
Urine, Escherichia coli, Antibiotic, AmpC, Beta-Lactamase
JOURNAL NAME:
Open Access Library Journal,
Vol.9 No.7,
July
14,
2022
ABSTRACT: Escherichia coli also known as E. coli are gram-negative facultative anaerobic, rod-shaped, Coliform bacteria, commonly found in the lower intestine of warm-blooded organisms. This study was designed to determine the antibiotic susceptibility and the phenotypic detection of AmpC beta-lactamase producing Escherichia coli from the urine of students attending FuLafia Clinic, Nasarawa State, Nigeria. A total of 22 urine samples were collected from the students. Eleven (50%) Escherichia coli isolates were recovered and identified by standard Microbiological methods. The antibiotic susceptibility of Escherichia coli from the urine of students showed that the isolates were susceptible to gentamycin (27%), streptomycin (22.7%), chloramphenicol (18%), sparfloxacin (13.6%), tarivid (9%), augumentin and septrin with (4.5%) each, while none was susceptible to amoxicillin/clavulanic acid, pefloxacin and ciprofloxacin respectively. The E. coli isolated from the urine of the students showed varying antibiotic-resistant phenotypes with SXTCH-SP-CPX-AM-AU-CN-PEF-OFX-S as the most common; with a percentage occurrence of 27.3%. The commonest Multiple Antibiotic Resistance indexes (MAR) of these isolates was 0.9 and the frequency of occurrence was 11 (50%). The E. coli isolated from the urine showed that 5 (45.5%) were resistant to cefoxitin, as 3 (60.0%) out of which were confirmed to be AmpC beta-lactamase producing E. coli.