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Trapnell, C., Roberts, A., Goff, L., Pertea, G., Kim, D., Kelley, D.R., et al. (2012) Differential Gene and Transcript Expression Analysis of RNA-seq Experiments with TopHat and Cufflinks. Nature Protocols, 7, 562-578.
https://doi.org/10.1038/nprot.2012.016

has been cited by the following article:

• TITLE: Transcriptional Features of Cattle Visceral and Subcutaneous Adipose Tissues: A Study of RNA-seq

  AUTHORS: Xiaoyan Duan, Yu Liu, Xiaoxiao Zhang, Haiyi Zhao

  KEYWORDS: Visceral Adipose Tissue, Subcutaneous Adipose Tissue, Transcriptional Features, Cattle

  JOURNAL NAME: Open Journal of Animal Sciences, Vol.12 No.3, July 12, 2022

  ABSTRACT: Visceral and subcutaneous are the two major types of bovine adipose tissues, and they show metabolic and functional differences according to their distribution, exploring the transcriptional features of visceral and subcutaneous adipose tissues is necessary. In the present study, we conducted RNA-seq analysis to compare the transcriptome between visceral (great omental) and subcutaneous (backfat) adipose tissues from Chinese Simmental cattle and validate them by qRT-PCR. We found that 5864 genes were differentially expressed between two tissues, including 2979 up-regulated and 2885 down-regulated in visceral adipose tissue. Functional analysis revealed a variety of differentially expressed genes (DEGs) involved in lipid metabolism and immune response processes. This may provide valuable information to further our understanding of the complexity of gene regulation governing the physiology of different fat depots. This work highlighted potential genes regulating lipid metabolism and immune responses; it may contribute to a better understanding of the metabolic and functional differences between visceral and subcutaneous adipose tissues.