TITLE:
Study on the Preparation and Characterization of Glycyrrhizic Acid Liposomes
AUTHORS:
Bing Wang, Xiang Luo, Jue Wang, Yibao Jin, Tiejie Wang, Xiean Yu, Guo Yin
KEYWORDS:
Glycyrrhizic Acid, Liposome, Orthogonal Design, Quality Evaluation
JOURNAL NAME:
Pharmacology & Pharmacy,
Vol.13 No.6,
June
30,
2022
ABSTRACT: The purpose of this study was to prepare glycyrrhizic acid nanoliposomes and evaluate the encapsulation efficiency and other properties of glycyrrhizic acid nanoliposomes, which would provide a reference for further research on Glycyrrhizic Acid in the treatment of liver diseases. Firstly, the preparation conditions of glycyrrhizic acid liposomes were optimized by the orthogonal design method. Then, glycyrrhizic acid liposomes were prepared by ultrasonic-film dispersion method and the encapsulation efficiency was determined by the HPLC method. Finally, the properties of glycyrrhizic acid nanoliposomes were also studied. The results showed that the optimal preparation conditions of liposomes were as follows: the ratio of drug to lipid was 1:30; Lecithin: cholesterol = 1:1; Hydration medium: pure water; Ultrasonic time: 120 s. The encapsulation efficiency of liposomes was about 90%. The final liposomes were round and uniform in distribution, with an average particle size of about 50 nm and absolute zeta potential of −28.9 mV. In this study, glycyrrhizic acid liposomes were prepared and the optimal preparation conditions were optimized. The encapsulation efficiency of the liposomes under the optimized conditions was determined. The evaluation of the morphology, size, particle size and stability of the liposomes was completed.