TITLE:
Callus Induction of Young Leaf Coconut cv. MATAG with Combination of 2,4-Dichlorophenoxyacetic Acid (2,4-D), α-Naphthalene Acetic Acid (NAA) and Benzyl Amino Purin (BAP)
AUTHORS:
Zuraida Ab Rahman, Sentoor Kumaran Govindasamy, Ahmad Ngalim, Nur Auni Syazalien Adlan, Nur Najwa Arifah Basiron, Ayu Nazreena Othman
KEYWORDS:
Callus Induction, Coconut, NAA, 2, 4-D
JOURNAL NAME:
Advances in Bioscience and Biotechnology,
Vol.13 No.5,
May
30,
2022
ABSTRACT: This research was to study in vitro callus induction
in Coconut cv MATAG from young leaf explants. Young leaf segments from mature
coconut were cultured on Y3 medium
supplemented with different concentrations of 2,4-D and a combination of
NAA and BAP. Each of these plant growth regulators (PGR) gives different
responses toward callus
formation, the percentage of explants producing callus, the percentage of
callus proliferation, and the morphology of callus. A series of different
concentrations were used for 2,4-D (1, 5, 10, 20, 40, 60, 100 mg/L), NAA (1, 3,
5 mg/L) and BAP (1, 3, 5 mg/L) respectively. The range of days of callus
formation using 2,4-D treatments is 7 - 12 months, while the 2,4-D combined with NAA is recorded
at 2 - 5 months. Despite the variety of different
months between these plant growth regulators for callus formation, the
percentages of explants producing callus and callus proliferation are
different. The highest percentage of explants producing callus (2.9%) was
observed at 2,4-D (40 mg/mL), followed by 2.7% at 2,4-D (10.0 mg/mL) with NAA
(1 mg/mL). At a concentration of 100 mg/mL of 2,4-D, the highest percentage of
callus proliferation was found, as well.