TITLE:
CD4+ T-Cell Count, Serum Zinc, Copper and Selenium Levels in HIV Sero-Positive Subjects on ART and ART Naïve Subjects in Port Harcourt, Nigeria
AUTHORS:
Donatus Onukwufor Onwuli, Helen Waribo-Anthony, Okwuchi Eberechukwu Anyalebechi, Chioma Ugochukwu
KEYWORDS:
Anti Retroviral Therapy (ART), Human Immunodeficiency Virus (HIV), Trace Elements, CD4 T-Cells
JOURNAL NAME:
Journal of Biosciences and Medicines,
Vol.10 No.5,
May
25,
2022
ABSTRACT: Background: HIV infection results in depletion of immunocompetent cells such as CD4+ T-cells. Trace elements such as Copper, Zinc and selenium are known to be involved in immune function. In recent times, HIV-positive patients are treated with antiretroviral therapy (ART), with significant progress. This study was aimed at evaluating CD4+ T-cells levels, serum Copper, Zinc and Selenium levels in HIV seropositive subjects on ART and ART naive subjects (HIV positive subjects that have not started ART treatment) in Rivers State, Nigeria. Methods: 150 subjects aged 20 to 79 years were recruited after informed consent. 70 subjects were HIV-positive on ART, 30 subjects were HIV-positive ART naïve subjects, while 50 subjects were apparently healthy subjects. Ten (10) milliliters of blood was collected using a standard venipuncture technique from each subject for the analysis of CD4 T-cells using BD fluorescent activated cell sorter (FACSC count), serum Copper and Zinc were analyzed colorimetrically using semi auto-analyzer WP 21E, while selenium was analyzed using atomic absorption spectrophotometer ELICO, SL173. Data generated were analyzed using Graph-Pad Prism version 8.0.2 and p Result: This study revealed a significant reduction in mean zinc, selenium and CD4+ T-cell level respectively (p = 0.0006; 0.0001; 0.0001) in HIV-Positive subjects on ART and ART naive. There was also a significant increase in mean serum copper level in the HIV-positive subject as compared to control subjects (p = 0.0001). ART treatment improved the CD4+ T cell count and serum levels of selenium and zinc; however, ART did not correct the imbalance. Furthermore, female subjects on ART have a significantly higher CD4+ T-cell count than the males (p Conclusion: Selenium and Zinc deficiency are associated with HIV disease despite the role of ART hence micronutrient supplementation is advised for HIV-positive subjects on ART.