TITLE:
Study on Hemostatic Effect of Thrombin Embedded with Different Concentration of Polypeptide RDAD16
AUTHORS:
Wei Su, Bailin Dai, Wu Wang, Jianxin Tang, Liang Ma
KEYWORDS:
Thrombin, RDAD16, Coagulation
JOURNAL NAME:
Journal of Biosciences and Medicines,
Vol.10 No.5,
May
18,
2022
ABSTRACT: Limiting blood loss following trauma is critical for survival and can reduce the risk of death from coagulopathy, infection, and multisystem organ failure. A hemostatic material with biocompatibility and effective hemostatic properties was developed to avoid blood loss. The thrombin spray was prepared by using the polypeptide RDAD16 as a stabilizer. The circular dichroism study showed that the chemical structure of the polypeptide did not change as a stabilizer. The atomic force microscope analysis showed that RDAD16 has formed a fibrous substance in the aqueous solution and could effectively encapsulate the thrombin. The simple coagulation experiment showed that the polypeptide RDAD16 could be the effective stability of the thrombin. The coagulant time of thrombin encapsulated by polypeptide RDAD16 was 14 s after 30 days which showed a significant difference compared with the control sample. Wound closure test in mice showed that thrombin embedded with Polypeptide RDAD16 does not affect wound closure.