TITLE:
An Alternative Tool for Determining Flavonoid Compounds in Markhamia tomentosa and Bunchosia glandulifera Using Digital Image Analysis
AUTHORS:
Luciana M. Santos, Antonio J. Demuner, Daiane E. Blank, Cristiane I. Cerceau, Iara F. Demuner, Marcela R. Coura, Maria J. M. Firmino, Marcelo H. Santos, Neusa F. Moura
KEYWORDS:
Markhamia tomentosa, Bunchosia glandulifera, PhotoMetrix®, Flavonoid, Smartphone
JOURNAL NAME:
Open Journal of Applied Sciences,
Vol.12 No.5,
May
18,
2022
ABSTRACT: Bunchosia glanduliera stands out because of the high
content of flavonoid compounds in the pulp, contributing to the antioxidant
potential of fruit extracts. Another plant species rich in flavonoid compounds is Markhamia tomentosa. However, to perform such an assay, a high-cost instrument is needed. To develop a simple and low-cost method for the determination of flavonoid compounds in M. tomentosa and B. glandulifera with PhotoMetrix®program application use pixels of digital imaging as an alternative
method and linear correlation techniques for univariate analysis implementing
systems of RGB colors (red, green, and
blue). To determine the total flavonoids, the reaction with ferric chloride and
quercetin was used as a control. For the acquisition of data or smartphones,
low-cost materials were used, demonstrating the applicability of this
analytical tool while comparing its cost to other analytical instrumentation.
The total flavonoid content was also determined using a spectrophotometry
technique in the visible ultraviolet spectrum (UV-Vis). The pulp of B. glandulifera showed the highest
content of flavonoid compounds. The content of flavonoid compounds found in the
fruit of B. glandulifera was 259.54
mg 100 g-1. In relation to the results found in the analysis of
total flavonoids of M. tomentosa can
be observed in the flower in natura has a higher content of these compounds. The use of PhotoMetrix® for the determination of flavonoid compounds in M. tomentosa and B. glandulifera reduced expense
and analysis time. The method is reproducible and efficient. The proposed
method can be adopted in different species.