TITLE:
Effects of Bacillus subtilis Var. Natto Products on Capillary Blood Flow in Healthy Subjects with Peripheral Coldness: A Double-Blind, Placebo-Controlled, Randomized Parallel Study
AUTHORS:
Chiharu Fujita, Yukio Usui, Masanori Inoue
KEYWORDS:
Peripheral Coldness, Blood Flow, Bacillus subtilis, Functional Foods
JOURNAL NAME:
Food and Nutrition Sciences,
Vol.13 No.3,
March
15,
2022
ABSTRACT: Purpose: NKCP®, a natto-derived dietary food supplement whose
main component is bacillopeptidase F produced by Bacillus subtilis var. natto, has antithrombotic, fibrinolytic, and pressure-lowering
effects, and also is suggested to improve peripheral coldness. However,
existing data are based on subjective evaluations with no scientific basis
about the effects on peripheral coldness. Therefore, we aimed to investigate
the effectiveness of NKCP® for peripheral coldness by measuring
changes in blood flow using a laser doppler rheometer and biochemical indices. Patients
and Methods: This was a double-blind, randomized, controlled study of
individuals aged 30 - 70 years who complained of subjective symptoms of cold hands and feet.
They were randomly divided into the NKCP® group and the placebo group
to receive NKCP® 250 mg once daily and dextrin 250 mg as placebo once
daily, respectively. The experiment lasted 8 weeks, with an intervention period
of 4 weeks and a washout period of 4 weeks. Results: One-month intake of NKCP®
significantly increased blood flow rate for 1 min between 4 and 5 minutes after
the end of cold loading compared to that before feeding (p = 0.038). Also,
analysis of the 5-minute blood flow rate before and after 4 weeks of feeding
showed a significant improvement in the NKCP® group (p = 0.007),
although there was no significant difference in the placebo group (p = 0.215).
Furthermore, the 5-minute blood flow at 4 weeks after the end of feeding was
significantly improved compared to that before feeding in the NKCP®
group (p = 0.049). Therefore, the effect continued for at least 1 month after
discontinuation of administration. Conclusions: It is possible that NKCP®
intake effectively improves blood flow in subjects with peripheral coldness.
Therefore, continuous intake of NKCP® is expected to reduce the
symptoms of peripheral coldness. In the future, it needs to investigate whether
the effect of increasing blood flow after ingestion of NKCP® is
effective in improving the symptoms of peripheral coldness.