TITLE:
The Frequency of rs1799889 in Plasminogen Activator Inhibitor Type-1 Gene in Sudanese Type 2 Diabetic Patients, Gezira State, Sudan, 2020-2021
AUTHORS:
Rowida Eljack Ibrahim, Sanaa Elfatih Hussein Ibrahim, Khalid Abdelsamea Mohamedahmed, Abdarahim Ali Babikir Haj Alzebar, Rania Ali Abdella Mohamed, Adil Mergani Babiker, Bakri Yousif Mohamed Nour
KEYWORDS:
4G/5G Polymorphism, Diabetic Mellitus Type 2 and PAI-1 Gene
JOURNAL NAME:
Open Journal of Applied Sciences,
Vol.12 No.2,
February
16,
2022
ABSTRACT: Background and Objectives: The cornerstone of the regulation
fibrinolytic system is plasminogen activator inhibitor type-1. The 4G/5G
polymorphism in the PAI-1 gene is a
key genetic predictor of increased plasma levels which is the most polymorphism
associated with cardiovascular complications. The 4G carriers have six times
higher PAI-1 levels than 5G carriers
leading to an increase in the level of plasma inhibitor by about 25% more than
5G allele (wide type). Type 2 diabetes presents symptoms of hypercoagulability
and hypofibrinolytic system that lead to contribute in the atherothrombosis and
then the myocardial infarction (MI). These findings supported the hypothesis
that there is a link between diabetes patients and this SNP. There is no data
about the prevalence of this allele in Sudanese diabetic patients with type 2
and the allele differs in prevalence according to ethnicity, for these reasons,
the aim of this study was to determine the allele and genotype frequency of the rs1799889 among Sudanese
T2DM patients. Methods: A case-control study was conducted using 70
diagnosed diabetes type 2 patients and 50 healthy individuals as the control
group. AS-PCR technique was used to genotype the rs1799889, and the
allelic frequency was calculated according to Hardy-Weinberg equilibrium.
Allelic frequencies were assessed using gene counting (SNP-STAT software V.
Release 3.13), and genotypes were scored. Results: The result showed
that 4G allele frequency was 28% among Sudanese diabetic patients without
statistical difference when compared with control group (P-value =
0.998) but, high when compared with other studies in African population 13% and
very low when compared with white and Indian populations studies. Conclusion: By this study, the allele frequency was higher in Sudanese diabetic patients with
type 2, and also we need another study to evaluate the effect of this
polymorphism in thrombophilic complications in Sudanese diabetic patients with type
2.