TITLE:
Selection of Tolerant of Some Citrus Hybrids F1 to Calcareous Stress and Identification Sexuall Individuals by SSR Marker
AUTHORS:
Venus Ebraheem Hasan, Ali Issa Elkhateeb, Hassan Yosef Khojah
KEYWORDS:
Citrus Rootstock, Calcareous Stress-Hybridization, SSR Markers-Proline, Chlorophyll
JOURNAL NAME:
American Journal of Plant Sciences,
Vol.13 No.1,
January
18,
2022
ABSTRACT: The research was conducted during two seasons
2018-2019 and 2019-2020, at the Scientific Research Center in Lattakia-Syria,
where the cross between Sour orange, Cleopatra mandarin and Trifoliate orange.
The first season was (Sour orange ♂ × Cleopatra
mandarin ♀) and (Cleopatra mandarin ♂ × sour orange ♀) and (sour orange ♂ × trifoliate orange ♀), and the crossing in the second season was (Trifoliate
orange ♂ × Sour orange ♀), and (Trifoliate orange ♂ × Cleopatra
mandarin ♀). The
plants of the first generation F1 were exposed to calcareous stress according
to two methods. The first one for a long term (two years) method was grown in
plots containing 15% and 35% Calcium Carbonate and the control free of Calcium
Carbonate for the first cross, and the second one short term (two months) by
watering method was with a solution of 1 mol and 2 mol of Calcium Carbonate for
the second cross. The indicators of proline and chlorophyll A, B and T were
measured in the first-generation individuals, once at the beginning of the
experiment and a second time at the end of study. Seven SSR primers were used
to identify sexual individuals, five primers were able to identify sexual F1
individuals are CSM13, CSM17, CSM147, CSM18 and TAA27, F1 individuals in the
first season (mixture experiment) gave high values of chlorophyll and were
tolerant to calcareous stress from crossing Cleopatra mandarin and Sour orange,
where they did not show a deterioration in chlorophyll, but rather increased
chlorophyll values, and the Trifoliate orange hybrids were sensitive to this
stress, and in the watering experiment the F1 showed significant deterioration
in Chlorophyll in all seedlings, and symptoms of Lime-induced chlorosis
appeared on the more sensitive seedlings.