TITLE:
Microbial Analysis of Drinking Water from Randomly Selected Boreholes and Shallow Wells around Hargeisa, Somaliland
AUTHORS:
Mohamud Ahmed Farah, Essam Abdulmuhsin Afifi, Nadira Harir Omar, Ahmed Hussein Essa
KEYWORDS:
Membrane Filtration Method, Escherichia Coli, Coliform, Shallow Wells, Boreholes, Indole, Colony Forming Unit, Indole, Citrate, Triple Sugar Iron
JOURNAL NAME:
Advances in Microbiology,
Vol.12 No.1,
December
31,
2021
ABSTRACT: Background: Shallow wells and boreholes are vital sources of potable water in Hargeisa. This water can be polluted by runoff, in particular during the rainy season, causing outbreaks of waterborne infections. Objectives: This research aimed at evaluating the microbial quality of shallow wells and boreholes water around Hargeisa, Somaliland. Methods: The total coliform and Escherichia coli count were done by using the membrane filtration method. Overall, 100 ml of each water sample was filtered via a 0.45 μm membrane filter, and then the filters were put on m-Endo agar plates that were incubated at 37°C for 24 to 48 hours. Results: The mean value of total coliform counts for the boreholes and shallow wells ranged from 1.288 × 103 to 8.8 × 103 CFU/100ml, while the mean value of total E. coli counts also ranged from 3.5 × 102 to 4.429 × 103 CFU/100ml. Results from this study have demonstrated that all water sources (Arabsiyo, Dararweyne, Darasalaam, Dabaraqas, and Jaleelo) don’t comply with the WHO guideline for drinking water. Results from the analysis of water samples of 28 wells demonstrated a significant correlation between total coliform and E. coli counts (P = 0.01). Therefore, this water is not fit for human consumption unless it is treated. Conclusion: This study has demonstrated that all results of both mean values of total coliform and E. coli counts from groundwater of selected shallow wells and boreholes were beyond WHO standards, so water from Arabsiyo, Jaleelo, Dabaraqas, Dararweyne, and Darasalaam requires treatment before human consumption.