TITLE:
Gamma Irradiation Enhanced Leaf Bioactive Components and Bioassay Parameters in M5 Mulberry (Morus Sp.) Mutant
AUTHORS:
Hyadalu Lingappa Ramesh, Lingenahalli Hosallappa Shivashankarappa, Veerapura Narayanappa Yoganandamurthy
KEYWORDS:
Bioassay, Cocoon, Commercial Characters, Gamma Rays, Genotype, Irradiation, Mulberry, M5, Phytochemical
JOURNAL NAME:
American Journal of Plant Sciences,
Vol.12 No.12,
December
29,
2021
ABSTRACT: Gamma radiation is an effective tool for inducing
genetic variation in plant characters. In the present experiment, M5 mulberry variety juvenile twigs were subjected to source Co60 gamma
irradiation (1 kR - 10 kR) and
mutants grown in triplicates in randomized block design to raise M1 and M2 generation. In M2 generation plants
were subjected to phytochemical and bioassay tests. Silkworm rearing parameters
and commercial characters of cocoons were recorded by feeding cross breed
silkworms. Results show that M5 mutant leaves revealed significant
variations in phytochemical constituents and moisture content. Bioassay tests
recorded significant differences compared to control in M2 generation. Commercial characters like cocoon weight (1.41 g), shell weight
(0.24 g), shell percentage (16.29 %), filament length (821.00 mts), renditta
(8.2), denier (2.24) and effective rate of rearing (92.14 %) were increased. It
is concluded that, gamma rays treatment
enhances the mulberry plants leaf bioactive components,
silkworm rearing and cocoon parameters and shows beneficial variants in
M2 generation.