TITLE:
Transcriptome Analysis of Gene in Mouse M-1 Cells Revealing the Functional Mechanism of Astragali Radix Extract
AUTHORS:
Bing Wang, Qian Feng, Chao Zhang, Yuming Chen, Mengmei Li, Yu Chen, Lin Wang, Jie Teng, Zhongzhen Cai, Yanpeng Zhao, Xuemei Bai
KEYWORDS:
Astragali Radix, RNA-Seq, Qi, Mitochondria
JOURNAL NAME:
Pharmacology & Pharmacy,
Vol.12 No.12,
December
24,
2021
ABSTRACT: Astragali Radix (AR), the dried root of
legumes, belongs to the Qi-invigorating herbs in traditional Chinese medicine
and plays an important role in the treatment of many diseases. In order
to understand the mechanism of action of AR extract. We used AR extract to
treat M-1, mouse kidney cells, and used transcriptome sequencing technology to detect
the genomic transcription level of the cells under the action of AR at
different concentrations and times. The results showed that after a low
concentration of AR treatments on the cells, the expression of genes related to
cell growth and cellular immune response changed significantly, among which
multiple genes are related to mitochondrial function, while high concentrations
of AR affected the expression of histones and disease-related genes. It showed
that the low concentration of AR extract can achieve the effect of invigorating
Qi by regulating the function of mitochondria. In addition, several important
genes and pathways were identified as potential targets of AR activation. The
research not only clarified the main molecular biological mechanism of AR
invigorating Qi, but also provided experimental basis and cellular physiology
reference for the further clinical application of AR.