TITLE:
Immobilization in Spheres of a Cocktail Rich in Xylanase Produced by the Fungus Fusarium sp. EA 1.3.1 for Hydrolysis of Sugarcane Bagasse
AUTHORS:
Alice Gomes Miranda, Tarcisio Michael Ferreira Soares de Oliveira, Rosymar Coutinho de Lucas, David Lee Nelson, Juan Pedro Bretas Roa, Vivian Machado Benassi
KEYWORDS:
Xylanase Cocktail, Spheres Immobilization, Lignocellulolytic Enzymes, Biomass Hydrolysis
JOURNAL NAME:
Advances in Bioscience and Biotechnology,
Vol.12 No.12,
December
17,
2021
ABSTRACT: Second generation ethanol is produced from the
degradation of lignocellulosic biomass using enzymes as catalysts, with
emphasis on xylanases. These biocatalysts are often costly, but stable at high
temperatures, and their reuse is of great value, so the immobilization of the
enzymes can increase their applicability on an industrial scale. We sought to
immobilize a cocktail rich in xylanase produced by the fungus Fusarium sp. EA 1.3.1 in alginate spheres, optimize
the immobilization method, characterize the immobilized derivatives, improve
their physical-chemical characteristics, and perform the hydrolysis of
sugarcane bagasse to release sugars. The Fusarium sp. EA 1.3.1 has been identified and used for cocktail rich in
xylanase production that was immobilized in alginate spheres. During this
process, the drip equipment, and the concentration of the solutions of sodium
alginate and calcium chloride were evaluated. The best results were obtained
with the glass rod and with concentrations of 3.14% and 2.10% for the solutions,
respectively. The apparent optimum conditions of pH and temperature reaction
were studied, and the values of pH 6.5 and 60°C were obtained. The
immobilized conjugate also presented greater stability at this temperature than
that of the soluble cocktail. The conjugate could be recycled up to six times,
and its activity was maintained after 75 days of storage. Finally, the
hydrolysis in natural sugarcane bagasse was achieved, and greater amounts of
reducing sugars were obtained in the reaction with the conjugate. Thus, the cocktail
rich in xylanase produced by the fungus Fusarium sp. EA1.3.1 was
successfully immobilized on alginate spheres and possesses the potential to be
used as a catalyst in industrial processes such as the lignocellulosic ethanol
industry.