TITLE:
Characterization of Magnaporthe oryzae triticum on Different Culture Media
AUTHORS:
M. L. Ashrafi, F. M. Aminuzzaman, M. K. Rehena, J. F. Tanni, M. Ahmed
KEYWORDS:
Wheat Blast, Culture Media, Magnaporthe oryzae triticum
JOURNAL NAME:
Open Access Library Journal,
Vol.8 No.12,
December
8,
2021
ABSTRACT: Wheat blast caused by the fungus Magnaporthe oryzae triticum (MoT) is one of the most devastating diseases of wheat. It has caused crop losses up to 40% to 100% in various wheat cultivars in different wheat growing regions. Understanding pathogenic variation, cultural and morphological characterization of MoT isolates is one of the most efficient ways to manage the disease. This study was carried out for isolation and pathogenicity of MoT isolates and evaluation of three culture media namely Oat Meal Agar (OMA), Potato Dextrose Agar (PDA) and Potato Sucrose Agar (PSA) for their growth and cultural characteristics. Wheat blast sample was collected from different fields in two districts during the Rabi cropping season in February to March 2019, in major wheat growing areas viz. Meherpur, Mujibnagar and Chuadanga sadar upazila. A total of 15 MoT isolates were identified and characterized based on their growth parameters. MoT isolates showed comparatively higher mean mycelial growth rate on OMA (15.43 mm per day) media followed by PDA (14.34 mm per day) at 4 DAI. Minimum mean mycelial growth rate was recorded on PSA (14.02 mm per day).