TITLE:
Investigation of the Effect of Rosmarinic Acid on Cyclophosphamide-Induced Gonadal Toxicity
AUTHORS:
Fırat Şahin, Fırat Aşır, Ebru Gökalp Özkorkmaz, Süreyya Özdemir Başaran, Özge Kaplan, Işılay Sezen Ermiş, Engin Deveci
KEYWORDS:
Testis, Cyclophosphamide, Rosmarinic Acid, Sertoli Cell, Hematoxylin Eosin
JOURNAL NAME:
Advances in Sexual Medicine,
Vol.12 No.1,
December
8,
2021
ABSTRACT: The aim
of this study was to investigate the effect of rosmarinic acid against gonadal
toxicity caused by cyclophosphamide, an important anticancer drug. A total of
28 rats were divided into 4 groups, with 7 animals in each group. The groups
were created as follows; Group 1 (control) (n = 7): Subjects received only 1 ml
of 0.9% saline solution per day intraperitoneally for 14 days. Group 2 (Rosmarinic
Acid) (n = 7): The subjects were given 20 mg/kg Rosmarinic acid
intraperitoneally for 14 days. Group 3 (Cyclophosphamide) (n = 7): Only 1 ml of
0.9% saline solution was administered intraperitoneally to the subjects for the
first 7 days. Cyclophosphamide 20 mg/kg per day was administered
intraperitoneally for the last 7 days (from the 8th day). Group 4
(Rosmarinic Acid +
Cyclophosphamide) (n = 7): The subjects were given 20 mg/kg Rosmarinic acid
daily intraperitoneally for 14 days. From the 8th day of the
experiment to the end of the experiment (last 7 days of the experiment), 20
mg/kg cyclophosphamide was given intraperitoneally daily. At the end of the
experiment, body weights of all rats were measured first. Afterwards, the
weights of testicular tissue samples were measured and the averages of the
weights were taken. Routine tissue follows up was performed on the testicular
tissues taken. Hematoxylin-eosin staining was applied to tissue sections of 5
μm thickness. As a result of the statistical analysis, it was determined that
cyclophosmadine decreased body and testicular weight, but rosmarinic acid had a
protective effect on the contrary. It was concluded that cyclophosmadine caused
damage to the basement membrane structure, Sertoli, Leydig and germ cells, but
these structures were preserved due to the protective effect of rosmarinic
acid. Despite the toxic effect of CP, rosmarinic acid is thought to have a
significant curative effect on the spermatogenetic process and seminiferous
tubule structure in the gonads.